Identification of the large-conductance background K+ channel in mouse B cells as TREK-2
- Authors
- Zheng, Haifeng; Nam, Joo Hyun; Pang, Bo; Shin, Dong Hoon; Kim, Ji Seon; Chun, Yang-Sook; Park, Jong-Wan; Bang, Hyowon; Kim, Woo Kyung; Earm, Yung E.; Kim, Sung Joon
- Issue Date
- Jul-2009
- Publisher
- AMER PHYSIOLOGICAL SOC
- Keywords
- K2P channel; arachidonic acid; PI kinase; membrane stretch; immune cells
- Citation
- AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v.297, no.1, pp C188 - C197
- Journal Title
- AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
- Volume
- 297
- Number
- 1
- Start Page
- C188
- End Page
- C197
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23129
- DOI
- 10.1152/ajpcell.00052.2009
- ISSN
- 0363-6143
1522-1563
- Abstract
- Zheng H, Nam JH, Pang B, Shin DH, Kim JS, Chun YS, Park JW, Bang H, Kim WK, Earm YE, Kim SJ. Identification of the large-conductance background K+ channel in mouse B cells as TREK-2. Am J Physiol Cell Physiol 297: C188-C197, 2009. First published May 13, 2009; doi:10.1152/ajpcell.00052.2009.-Mouse B cells and their cell line (WEHI-231) express large-conductance background K+ channels (LKbg) that are activated by arachidonic acids, characteristics similar to TREK-2. However, there is no evidence to identify the molecular nature of LKbg; some properties of LKbg were partly different from the reported results of TREK type channels. In this study, we compared the properties of cloned TREK-2 and LKbg in terms of their sensitivities to ATP, phosphatidylinositol 4,5-bisphosphate (PIP2), intracellular pH (pH(i)), and membrane stretch. Similar to the previous findings of LKbg, TREK-2 showed spontaneous activation after membrane excision (i-o patch) and were inhibited by MgATP or by PIP2. The inhibition by MgATP was prevented by wortmannin, suggesting membrane-delimited regulation of TREKs by phosphoinositide (PI) kinase. The same was observed with the property of LKbg; the activation of TREK-2 by membrane stretch was suppressed by U73122 (PLC inhibitor). As with the known properties of TREK-2, LKbg were activated by acidic pH(i) and inhibited by PKC activator. Finally, we confirmed the expression of TREK-2 in WEHI-231 by using RT-PCR and immunoblot analyses. The amplitude of background K+ current and the TREK-2 expression in WEHI-231 were commonly decreased by genetic knockdown of TREK-2 using small interfering RNA. The downregulation of TREK-2 attenuated Ca2+-influx induced by arachidonic acid in WEHI-231. As a whole, these results strongly indicate that TREK-2 encodes LKbg in mouse B cells. We also newly suggest that the low activity of TREK-2 in intact cells is due to the inhibition by intrinsic PIP2.
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