Free radical scavenging activity of butanol fraction from the fruit of citrus junosFree Radical Scavenging Activity of Butanol Fraction from the Fruit of Citrus junos
- Authors
- Cho, E.J.; Li, L.; Piao, X.-L.; Lee, S.; Kim, H.Y.
- Issue Date
- 2009
- Publisher
- 한국식품영양과학회
- Keywords
- Citrus junos; Free radical; Hydroxyl radicals; Superoxide anion
- Citation
- Journal of Food Science and Nutrition, v.14, no.1, pp 86 - 89
- Pages
- 4
- Journal Title
- Journal of Food Science and Nutrition
- Volume
- 14
- Number
- 1
- Start Page
- 86
- End Page
- 89
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23449
- DOI
- 10.3746/jfn.2009.14.1.086
- ISSN
- 1226-332X
2287-8602
- Abstract
- In this study, we investigated the free radical [1,1-diphenyl-2- picrylhydrazyl (DPPH), hydroxyl radical (.OH) and superoxide anion (O 2 -)] scavenging activity of MeOH extract and 3 fractions of Citrus junos. Of the tested fractions, the BuOH fraction showed the strongest DPPH scavenging activity, showing the IC50 values of 63.4 mg/mL. Therefore, we continuously carried out DPPH, .OH and O2 - scavenging activity tests of BuOH fraction of Citrus junos. The BuOH fraction of Citrus junos inhibited DPPH radical to 97.5% at a concentration of 1000 mg/mL and the scavenging activities were increased concentration-dependently. In addition, BuOH fraction from Citrus junos also scavenged .OH in a concentration dependent manner from 5 to 1000 mg/mL. Furthermore, BuOH fraction showed about 56% O2 -scavengimg activity at 25 mg/mL concentration but, the scavenging activities were not enhanced in a dose dependent manner. The present results suggest that BuOH fraction of Citrus junos would have the protective potential from oxidative stress induced by free radicals. Copyright © 2007 KFN.
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