Calcium Hydroxide Inactivates Lipoteichoic Acid from Enterococcus faecalis
- Authors
- Baik, Jung Eun; Kum, Kee-Yeon; Yun, Cheol-Heui; Lee, Jin-Kyung; Lee, Kangseok; Kim, Kack Kyun; Han, Seung Hyun
- Issue Date
- Nov-2008
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Apical periodontitis; calcium hydroxide; endodontic medicament; Enterococcus faecalis; lipoteichoic acid; Toll-like receptor 2
- Citation
- JOURNAL OF ENDODONTICS, v.34, no.11, pp 1355 - 1359
- Pages
- 5
- Journal Title
- JOURNAL OF ENDODONTICS
- Volume
- 34
- Number
- 11
- Start Page
- 1355
- End Page
- 1359
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23576
- DOI
- 10.1016/j.joen.2008.08.014
- ISSN
- 0099-2399
1878-3554
- Abstract
- Calcium hydroxide is a widely used endodontic medicament for eliminating viable bacteria and inactivating virulence factors. Enterococcus faecalis, a pathogenic gram-positive bacterium, has been associated with refractory apical periodontitis, Because lipoteichoic acid (LTA) is a major virulence factor of gram-positive bacteria, we examined whether calcium hydroxide could detoxify LTA from E. faecalis. An enzyme-linked immunosorbent assay showed that calcium hydroxide-killed E. faecalis was less potent than heat-killed bacteria in stimulating the release of tumor necrosis factor-a by a murine macrophage line, RAW 264.7 (P < 0.05). Pretreatment of LTA with calcium hydroxide remarkably abrogated the ability of LTA to induce the release of tumor necrosis factor-alpha (P < 0.05). Furthermore, calcium hydroxide-treated LTA was not able to stimulate Toll-like receptor 2, which recognizes functionally intact LTA. These results suggest that calcium hydroxide could detoxify LTA, resulting in attenuation of the inflammatory responses to E. faecalis and its LTA. (J Endod 2008;34:1355-1359)
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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