Characterization of nuclear localization signals of the prototype foamy virus integrase
- Authors
- An, Dog Gn; Hyun, Usok; Shin, Cha-Gyun
- Issue Date
- Jul-2008
- Publisher
- SOC GENERAL MICROBIOLOGY
- Citation
- JOURNAL OF GENERAL VIROLOGY, v.89, no.7, pp 1680 - 1684
- Pages
- 5
- Journal Title
- JOURNAL OF GENERAL VIROLOGY
- Volume
- 89
- Number
- 7
- Start Page
- 1680
- End Page
- 1684
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23700
- DOI
- 10.1099/vir.0.83689-0
- ISSN
- 0022-1317
1465-2099
- Abstract
- To analyse the potential karyophilic activity of prototype foamy viruses (PFVs), we expressed the PFV integrase (IN) and its mutants as fusion proteins with enhanced green fluorescence protein. The subcellular localization of the fusion proteins was investigated by fluorescence microscopy. The PFV IN was found to be karyophilic and targeted the fusion protein to the nucleus. Mutational analyses demonstrated that the PFV IN contains a potent but non-transferable nuclear localization signal (NLS) in its C-terminal domain and contains five arginine and lysine residues between amino acids 308 and 329 that are critical for its NLS function.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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