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Technical note: improved ancient DNA purification for PCR using ion-exchange columns
- Authors
- Kim, Kijeong; Kim, Kyung-Yong; Jeon, Eunhee; Togloom, Ariunaa; Cho, Youn-Ock; Lee, Min-Soo; Lkhagvasuren, Gavaachimed; Choi, Jee-Hye; Tumen, Dashtseveg; Park, Ae Ja; Kim, Keun-Cheol; Park, Ki-Won; Kim, Jae-Hyun; Noh, Maengseok; Yoo, Kwon-Jong; Lee, Kwang-Ho
- Issue Date
- May-2008
- Publisher
- WILEY
- Keywords
- DNA purification; ancient bone; PCR amplification; ion-exchange columns; PCR inhibitors
- Citation
- AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, v.136, no.1, pp 114 - 121
- Pages
- 8
- Journal Title
- AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY
- Volume
- 136
- Number
- 1
- Start Page
- 114
- End Page
- 121
- ISSN
- 0002-9483
1096-8644
- Abstract
- A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500-3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples.
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