Abundance of aryl hydrocarbon receptor potentiates benzo[a]pyrene-induced apoptosis in Hepa1c1c7 cells via CYP1A1 activation
- Authors
- Chung, Jin-Yong; Kim, Ji Young; Kim, Won Rok; Lee, Seung Gee; Kim, Yoon-Jae; Park, Ji-Eun; Hong, Yeon-Pyo; Chun, Young-Jin; Park, Young Chul; Oh, Seunghoon; Yoo, Ki Soo; Yoo, Young Hyun; Kim, Jong-Min
- Issue Date
- Jun-2007
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- AhR; CYP1A1; CYP1B1; benzo[a]pyrene; hepatoma cells; apoptosis
- Citation
- TOXICOLOGY, v.235, no.1-2, pp 62 - 72
- Pages
- 11
- Journal Title
- TOXICOLOGY
- Volume
- 235
- Number
- 1-2
- Start Page
- 62
- End Page
- 72
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24053
- DOI
- 10.1016/j.tox.2007.03.013
- ISSN
- 0300-483X
- Abstract
- Although B[a]P-induced apoptosis has been demonstrated in Hepa1c1c7 cells, the cellular signaling pathway(s) by which benzo[a]pyrene (B[a]P) elicits a cytotoxicity-mediated apoptogenic role remains to be elucidated. In this study, we showed that B[a]P induces apoptosis in a p53-mediated and caspase- 3 -dependent manner, which relates to the accumulation of the S phase of the cell cycle. Importantly, we have shown for the first time that Hepa1c1c7 cells retain a considerably high content of aryl hydrocarbon receptor (AhR) protein before B[a]P exposure, assuming that this status enables the cells to respond to B[a]P more readily as well as more efficiently. B[a]P treatment resulted in the downregulation of AhR and induced cytochrome P450 1A1 (CYP1A1) (but not cytochrome P450 1B1 (CYP1B1)) expression and activity. While (alpha-naphtoflavone (alpha-NF) and ellipticine suppressed B[a]P-induced CYP1A1 activation as well as apoptosis, the 2,3,4,5'-tetramethoxystilbene (TMS) and pyrene, known CYP1B1 inhibitors, failed to inhibit apoptosis. However, alpha-NF alone significantly increased CYP1A1 protein expression but not its activity, suggesting that alpha-NF more likely works as an AhR agonist in Hepa1c1c7 cells after B[a]P, rather than a direct inhibitor of CYP1A1 activity. In conclusion, it is suggested that the abundance of endogenous AhR level is an indispensable condition for an efficient cellular signaling of B[a]P and that control of AhR activity in Hepa1c1c7 cells might be important to cell fate resulting from CYP1A1 activation after B[a]P. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
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