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Detection of methicillin resistance in Staphylococcus aureus isolates using two-step triplex PCR and conventional methods
- Issue Date
- Apr-2007
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- two-step triplex PCR; methicillin-resistant S. aureus (MRSA); mecA gene; femA gene; nuc gene
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.4, pp 673 - 676
- Pages
- 4
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 17
- Number
- 4
- Start Page
- 673
- End Page
- 676
- ISSN
- 1017-7825
1738-8872
- Abstract
- A two-step triplex PCR assay targeting the mecA, femA, and mic genes was developed for the detection of methicillin resistance genes harbored by some Staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. The triplex PCR revealed the presence of the jemA and nuc genes in all the S. aurelis isolates examined (n=105). Forty-four clinical isolates were mecA positive and no foodborne isolates were mecA positive. The PCR results had a 98 or 99% correlation with the results of PBP2a latex agglutination tests or oxacillin susceptibility tests, respectively.
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