Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection
- Authors
- Um, SY; Jung, SH; Jung, SJ; Kim, JI; Chung, SY; Lee, HJ; Han, SB; Choi, SO
- Issue Date
- Jun-2006
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- fluvastatin; column-switching; high-performance liquid chromatography (HPLC); rat plasma; direct injection
- Citation
- JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.41, no.4, pp 1458 - 1462
- Pages
- 5
- Journal Title
- JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
- Volume
- 41
- Number
- 4
- Start Page
- 1458
- End Page
- 1462
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24325
- DOI
- 10.1016/j.jpba.2006.03.021
- ISSN
- 0731-7085
1873-264X
- Abstract
- A column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of fluvastatin in rat plasma. Plasma samples were diluted with an equal volume of mobile phase, i.e. acetonitrile-5 mM potassium phosphate buffer (pH 6.8) (15:85, v/v), and the mixture was directly injected onto the HPLC system. The analyte was enriched in a pre-treatment column, while endogenous components were eluted to waste. The analyte was then back-flushed onto an analytical column and quantified with fluorescence detection (lambda(ex) = 305 nm; lambda(em) = 390 nm). The standard curve for the drug was linear in the range 0.5-100 ng mL(-1) in rat plasma. The limit of quantitation for plasma was found to be 0.5 ng mL(-1). This method has been fully validated and shown to be specific, accurate and precise. The method is simple and rapid because of a minimized sample preparation and appears to be useful for the pharmacokinetic study of fluvastatin. (c) 2006 Published by Elsevier B.V.
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