Expression and modulation of LL-37 in normal human keratinocytes, HaCaT cells, and inflammatory skin diseases
- Authors
- Kim, JE; Kim, BJ; Jeong, MS; Seo, SJ; Kim, MN; Hong, CK; Ro, BI
- Issue Date
- Aug-2005
- Publisher
- KOREAN ACAD MEDICAL SCIENCES
- Keywords
- CAP18 lipopolysaccharide-binding protein; LL-37; keratinocytes; HaCaT cell; psoriasis; dermatitis; atopic
- Citation
- JOURNAL OF KOREAN MEDICAL SCIENCE, v.20, no.4, pp 649 - 654
- Pages
- 6
- Journal Title
- JOURNAL OF KOREAN MEDICAL SCIENCE
- Volume
- 20
- Number
- 4
- Start Page
- 649
- End Page
- 654
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24553
- DOI
- 10.3346/jkms.2005.20.4.649
- ISSN
- 1011-8934
1598-6357
- Abstract
- Defensins and cathelicidins (LL-37) are major antimicrobial peptides (AMPs) of the innate immune system of the human skin. In normal non-inflamed skin these peptides are negligible, but their expression can be markedly increased in inflammatory skin disease such as psoriasis. We designed this study to identify the expressions of LL-37 in normal human keratinocyte (NHK) and HaCaT cells after exposure to stimulants and to investigate difference of LL-37 expression accompanied with cell differentiation status, and come to understand difference of susceptibility to infection in atopic dermatitis and psoriasis. Expressions of LL-37 in NHKs and HaCaT cells were evaluated by using RT-PCR, Western blotting, and immunohistochemical (IHC) staining at 6, 12, and 24 hr post stimulation after exposure to Ultraviolet B irradiation and lipopolysaccharide. And expression of LL-37 in skin biopsy specimens from patients with atopic dermatitis and psoriasis was determined by immunohistochemical analysis. In time-sequential analyses of LL-37 expression revealed that LL-37 was expressed in NHKs, but not in HaCaT cells. IHC analysis confirmed the presence of abundant LL-37 in the epidermis of psoriasis. Therefore we deduced that expression of LL-37 is affected by UV irradiation, bacterial infection, and status of cell differentiation.
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