Relaxation of rabbit cavernous smooth muscle to 17 beta estradiol: a non-genomic, NO-independent mechanism
- Authors
- Kim, Sae-Chul; Seo, Kyung-Kun; Myung, Soon-Chul; Lee, Moo Yeol
- Issue Date
- Jun-2004
- Publisher
- SCIENCE CHINA PRESS
- Keywords
- 17 beta-estradiol; cavernous smooth muscles; Maxi-K channel; relaxation
- Citation
- ASIAN JOURNAL OF ANDROLOGY, v.6, no.2, pp 127 - 131
- Pages
- 5
- Journal Title
- ASIAN JOURNAL OF ANDROLOGY
- Volume
- 6
- Number
- 2
- Start Page
- 127
- End Page
- 131
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24841
- ISSN
- 1008-682X
1745-7262
- Abstract
- Aim: To investigate whether estrogen was involved in relaxation of rabbit cavernous smooth muscle. Methods: Relaxation response of the rabbit cavernous smooth muscles to 17beta-estradiol (0.3, 3, 30 and 300 nmol/L) were observed in vitro. The response of the muscle strips to estrogen after incubation with either actinomycin D (10 mumol/L) or L-NAME (10 mumol/L) were also evaluated. Inside-out mode of patch clamp in a single smooth muscle cell was applied to investigate the Maxi-K channel activities. Results: Estrogen caused a dose-dependent relaxation of the strips precontracted with norepinephrine. The maximal response was noted about 10 minutes after treatment. The estrogen-induced relaxation was prevented by neither actinomycin D nor L-NAME, suggesting that the response was not mediated by gene transcription or nitric oxide (NO). Application of 17beta-estradiol increased the Maxi-K channel activities. Conclusion: 17beta-estradiol may be involved in relaxation of rabbit cavernous smooth muscles via a non-genomic and NO independent mechanism. 17beta-estradiol stimulates Maxi-K channel of the rabbit cavernous myocyte.
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