Use of internal standard RNA molecules for the RT-PCR amplification of the faeces-borne RNA viruses
- Authors
- Kim, K; Park, J; Chung, Y; Cheon, D; Lee, IB; Lee, S; Yoon, J; Cho, H; Song, C; Lee, KH
- Issue Date
- Jul-2002
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- internal standard RNA; RT-PCR; faeces-borne viruses; diagnosis
- Citation
- JOURNAL OF VIROLOGICAL METHODS, v.104, no.2, pp 107 - 115
- Pages
- 9
- Journal Title
- JOURNAL OF VIROLOGICAL METHODS
- Volume
- 104
- Number
- 2
- Start Page
- 107
- End Page
- 115
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25104
- DOI
- 10.1016/S0166-0934(02)00016-2
- ISSN
- 0166-0934
1879-0984
- Abstract
- The diagnostic system based on reverse transcription (RT)-PCR has been used widely for the detection of viral genomes of faecal-borne RNA viruses. However, faecal specimens often produce both false positive and false negative results. Therefore, there is a need for a diagnosis procedure that can control for 'false-results'. In this study, an internal standard RNA that can serve as a non-competitive positive template was developed and used directly to detect faecal-borne RNA viruses without noticeable competitive inhibition of the target viral genome. These results suggest that the internal standard RNA is a useful standard molecule when undertaking diagnostic qualitative RT-PCR procedures for enteroviruses and related faecal-borne RNA viruses. (C) 2002 Elsevier Science B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25104)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.