Bax-dependent apoptosis induced by ceramide in HL-60 cells
- Authors
- Kim, HJ; Mun, JY; Chun, YJ; Choi, KH; Kim, MY
- Issue Date
- Sep-2001
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- ceramide; Bax; Bcl-xL; apoptosis; caspase
- Citation
- FEBS LETTERS, v.505, no.2, pp 264 - 268
- Pages
- 5
- Journal Title
- FEBS LETTERS
- Volume
- 505
- Number
- 2
- Start Page
- 264
- End Page
- 268
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25183
- DOI
- 10.1016/S0014-5793(01)02836-8
- ISSN
- 0014-5793
1873-3468
- Abstract
- Ceramide is an important lipid messenger involved in mediating a variety of cell functions including apoptosis. In this study, we show that antisense bax inhibits cytochrome c release, poly(ADP-ribose)polymerase cleavage and cell death induced by ceramide in HL-60 cells. In addition, ceramide induces translocation of Bax to mitochondria. The addition of the broad spectrum caspase inhibitor zVAD-fmk prevented ceramide-induced apoptotic cell death but did not inhibit translocation of Bax and mitochondrial cytochrome c release. Furthermore, ceramide inhibits the expression of the antiapoptotic protein Bcl-xL with an increase in the ratio of Bax to Bcl-xL. These data provide direct evidence that Bax plays an important role in regulating ceramide-induced apoptosis. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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