The involvement of phospholipase A(2) in ethanol-induced gastric muscle contraction
- Authors
- Sim, SS; Choi, JC; Min, DS; Rhie, DJ; Yoon, SH; Hahn, SJ; Kim, CJ; Kim, MS; Jo, YH
- Issue Date
- Feb-2001
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- cyclooxygenase; ethanol; lipoxygenase; phospholipase A(2); phospholipase C; smooth muscle
- Citation
- EUROPEAN JOURNAL OF PHARMACOLOGY, v.413, no.2-3, pp 281 - 285
- Pages
- 5
- Journal Title
- EUROPEAN JOURNAL OF PHARMACOLOGY
- Volume
- 413
- Number
- 2-3
- Start Page
- 281
- End Page
- 285
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25240
- DOI
- 10.1016/S0014-2999(01)00753-1
- ISSN
- 0014-2999
1879-0712
- Abstract
- To understand the underlying mechanism of ethanol in tonic contraction, the effect of ethanol on phospholipase A(2) and phospholipase C activities and the effects of phospholipase inhibitors on ethanol-induced contraction of cat gastric smooth muscle were tested. Circular muscle strips (2.0 x 0.2 cm) obtained from the fundus of cat stomach were used to measure isometric contraction. Ethanol elicited tonic contraction and activated phospholipase A(2) activity in a dose-dependent manner. Phospholipase A(2) inhibitors, manoalide (0.1-10 muM) and oleyloxyethyl phosphorylcholine (1-10 muM), significantly inhibited ethanol-induced contraction. Furthermore, 342 mM ethanol-induced contraction was significantly inhibited by cyclooxygenase inhibitors, ibuprofen (10-100 muM) and indomethacin (10-100 muM), but not by lipoxygenase inhibitors. On the other hand phospholipase C inhibitors had no effect on ethanol-induced contraction, indicating that phospholipase C is not involved in ethanol-induced contraction. It is suggested from the above results that ethanol-induced contraction in cat gastric smooth muscle is, in part, mediated by phospholipase A(2) and cyclooxygenase pathways. (C) 2001 Elsevier Science B.V. All rights reserved.
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