Differential effects of nitric oxide synthase inhibitors in rats
- Authors
- Lee, J.-H.; Chang Yell Shin; Bong Su Kang; Jeong, Ji Hoon; Kyeong Bum Choi; Young Sil Min; Jin Hak Kim; In Hoi Huh; Uy Dong Sohn
- Issue Date
- 2000
- Keywords
- N(G)-monomethyl-L-arginine; N(G)- nitro-L-arginine methylester; Nerve conduction velocity; Nitric oxide; Platelet activating factor; Superoxide anion
- Citation
- Korean Journal of Physiology and Pharmacology, v.4, no.2, pp 99 - 104
- Pages
- 6
- Journal Title
- Korean Journal of Physiology and Pharmacology
- Volume
- 4
- Number
- 2
- Start Page
- 99
- End Page
- 104
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25589
- ISSN
- 1226-4512
2093-3827
- Abstract
- We investigated the action of NOS inhibitors on NOS in rots. Both of nitric oxide synthase inhibitors, N(G)-monomethyl-L-arginine (L-NMMA, 3 μM) or N(G)-nitro-L-arginine methylester (L-NAME, 30 μM), augmented phenylephrine (PE, 10-7 M)-induced contraction which was inhibited by acetylcholine (ACh) in rat thoracic aorta. This augmentation by L-NAME or L- NMMA was attenuated with the treatment of NO precursor, arginine. ACh, however, decreased the augmentation induced by L-NMMA, but not by L-NAME. Superoxide dismutase (SOD, 50 u/ml) potentiated an inhibitory effect of ACh on the PE (10-7 M)-induced contraction. It has been known that platelet activating factor itself induces iNOS. Platelet activating factor (PAF, 10- 7 M) inhibited PE (10-7 M)-induced contraction. Pretreatment with L-NMMA (30 mM) or L-NAME (30 mM) significantly blocked the inhibitory action of PAF on PE-induced contraction. L-NMMA (100 mM) or L-NAME (100 mM) reduced nerve conduction velocity (NCV) relevant to nNOS in rat sciatic nerve. ACh attenuated the reduction of NCV by L-NMMA-, but not by L-NAME-induced reduction of NCV. These results suggest that L-NMMA and/or L-NAME have different action on three types of NOS in rats.
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