Roseicella frigidaeris gen. nov., sp. nov., isolated from an air-conditioning system

Abstract

A Gram-stain-negative, facultatively aerobic bacterial strain, designated DB1506(T), of the family Acetobacteraceae, was isolated from an air-conditioning system in the Republic of Korea. Colonies were pink- to rosy-coloured and cells were nonmotile cocci with catalase- and oxidase-positive activities. Growth of strain DB1506(T) was observed at 20-37 degrees C (optimum, 30 degrees C), pH 5.5-8.5 (pH 7.0) and in the presence of 0-0.5 % (w/v) NaCl (0 %). Strain DB1506(T) contained summed feature 8 (comprising C-18(:1)omega 7c and/or C-18:(1)omega 6c) and C-18(:1) 2-OH as major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipids, unidentified aminolipids and unidentified polar lipids were detected as major polar lipids. The G+C content of the genomic DNA calculated from the whole genome sequence was 72.5 mol%. Strain DB1506(T) was most closely related to Paracraurococcus ruber NS89(T), Dankookia rubra WS-10(T) and Siccirubricoccus deserti SYSU D8009(T) with 16S rRNA gene sequence similarities of 96.01, 95.88 and 95.44%, respectively, but strain DB1506(T) formed a clearly distinct phylogenic lineage from them within the family Acetobacteraceae. On the basis of phenotypic, chemotaxonomic and molecular properties, strain DB1506(T) represents a novel species of a new genus within the family Acetobacteraceae, for which the name Roseicella frigidaeris gen nov., sp nov. is proposed. The type strain is DB1506(T) (=KACC 19791(T)=JCM 32945(T)).