Paraburkholderia lacunae sp. nov., isolated from soil near an artificial pond

Abstract

A Gram-stain-negative, strictly aerobic bacterial strain, designated strain S27(T), was isolated from soil near an artificial pond in South Korea. Cells were non-motile short rods showing oxidase- and catalase-positive activities. Growth of strain S27(T) was observed at 20-40 degrees C (optimum, 30 degrees C), pH 5.0-7.0 (optimum, pH 6.0), and 0-0.5% (w/v) NaCl (optimum, 0%). Ubiquinone-8 was detected as the sole respiratory quinone and the major fatty acids were C-16:0, cyclo-C-17:0, and cyclo-C(19:0)8c. The G + C content of the genomic DNA was 62.4 mol%. Phosphatidylglycerol, phosphatidylethanolamine, and an unidentified aminophospholipid were detected as the major polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S27(T) formed a clearly distinct phyletic lineage from closely related Paraburkholderia species within the genus Paraburkholderia. Strain S27(T) was most closely related to Paraburkholderia rhynchosiae WSM3937(T), Paraburkholderia ginsengiterrae DCY85(T), Paraburkholderia fungorum NBRC 102489(T), and Paraburkholderia graminis C4D1M(T) with 98.8%, 98.4%, 98.4%, and 97.7% 16S rRNA gene sequence similarities, respectively. The DNA-DNA relatedness level between strain S27(T) and the type strain of P. rhynchosiae was 36.8 +/- 2.6%. On the basis of phenotypic, chemotaxonomic and molecular properties, strain S27(T) represents a novel species of the genus Paraburkholderia, for which the name Paraburkholderia lacunae sp. nov. is proposed. The type strain is S27(T) (KACC 19714 (T) = JCM 32721(T)).