Microcontact printing of biotin for selective immobilization of streptavidin-fused proteins and SPR analysis
- Authors
- Park, Jong Pil; Lee, Seok Jae; Park, Tae Jung; Lee, Kyung-Bok; Choi, Insung S.; Lee, Sang Yup; Kim, Min-Gon; Chung, Bong Hyun
- Issue Date
- Apr-2004
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- microcontact printing (mu CP); pattern generation; protein-protein assay; surface plasmon resonance
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.9, no.2, pp 137 - 142
- Pages
- 6
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 9
- Number
- 2
- Start Page
- 137
- End Page
- 142
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/27636
- DOI
- 10.1007/BF02932997
- ISSN
- 1226-8372
- Abstract
- In this study, a simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (CP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Confocal fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. The results suggest that this strategy, which consists of a combination of muCP and cSA-fused proteins, is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications, one such being the use in protein-protein assays.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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