Advanced cleanup process of the free-flow microfluidic device for protein analysis
- Authors
- Huh, Yun Suk; Park, Tae Jung; Yang, KwangSuk; Lee, Eun Zoo; Hong, Yeon Ki; Lee, Sang Yup; Kim, Do Hyun; Hong, Won Hi
- Issue Date
- Sep-2008
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- electro-desalting; microfluidic device; sample preparation; fusion protein; biosensor
- Citation
- ULTRAMICROSCOPY, v.108, no.10, pp 1365 - 1370
- Pages
- 6
- Journal Title
- ULTRAMICROSCOPY
- Volume
- 108
- Number
- 10
- Start Page
- 1365
- End Page
- 1370
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/27662
- DOI
- 10.1016/j.ultramic.2008.04.085
- ISSN
- 0304-3991
1879-2723
- Abstract
- The treatment of samples preparation is generally recognized as a bottleneck for the rapid analysis of protein because of the off-chip performance in many cases. In this study, we used the charge characteristics of protein to develop a simple and rapid electro-microfluidic desalting system as an effective means of cleaning up protein sample. When we loaded a urea-rich protein sample and a buffer solution into a free-flow zone electrophoresis (FFZE) chamber, the microfluidic device was able to separate the charged protein sample and the non-charged urea. With a 90V electric field in the FFZE chamber, the removal efficiency of the urea was about 88% and the recovery of the protein was 78%. In addition, the desalted protein sample used in this device showed significant improvement with respect to the MALDI-TOF-MS spectrum signal of a fusion protein, which was fused to the gold-binding polypeptide with enhanced green fluorescent protein, as a model protein. The inflow of the purified fusion protein sample can be successfully immobilized on the gold surface and analyzed by confocal fluorescence microscopy and surface plasmon resonance for biotechnological sensors. (c) 2008 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.