Graphene-based electrochemical biosensor for pathogenic virus detection
- Authors
- Liu, Fei; Choi, Ki Seok; Park, Tae Jung; Lee, Sang Yup; Seo, Tae Seok
- Issue Date
- Jun-2011
- Publisher
- SPRINGER
- Keywords
- Graphene film; Electrochemical biosensor; Pathogen detection; Detection sensitivity
- Citation
- BIOCHIP JOURNAL, v.5, no.2, pp 123 - 128
- Pages
- 6
- Journal Title
- BIOCHIP JOURNAL
- Volume
- 5
- Number
- 2
- Start Page
- 123
- End Page
- 128
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/27776
- DOI
- 10.1007/s13206-011-5204-2
- ISSN
- 1976-0280
2092-7843
- Abstract
- A graphene based biosensor system is presented for performing highly sensitive pathogenic virus detection. A free-standing conductive graphene film was prepared as a novel electrochemical sensor through two steps: synthesis of a graphene oxide (GO) film from GO colloidal suspensions by using a speed vacuum concentrator and thermal annealing process at 900 degrees C with H(2)/Ar flow to generate a reduced GO film. The resultant graphene film shows an excellent electron transfer property on the surface in the [Fe(CN)(6)](3-)/(4-) redox system and is used as a working electrode in the electrochemical biosensor. The surface of graphene is modified with pyrene derivatives, and then covalently linked with virus-specific antibodies. The target cell, rotavirus, is captured on the graphene film through antibody-antigen interaction, and the entire process was monitored by cyclic voltammetric responses. A 10(5) pfu/mL of input cells is detected with ca. 30.7% sensitivity, and ca. 1.3% sensitivity is measured with 10(3) pfu/mL of input cells, demonstrating that graphene film based electrode can be applied for electrochemical biosensor.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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