Urethane으로 유발된 생쥐 폐샘암종 발생과정에서 세포주기 관련인자 (Cyclin D1, p21, and p27)에 대한 비소의 효과Effects of Arsenic Trioxide on Cell Cycle Related Proteins (Cyclin D1, p21, p27) Expression DuringUrethane-induced Lung Carcinogenesis in Mice
- Authors
- 이성혁; 정지훈; 견종만; 박언섭
- Issue Date
- 2006
- Publisher
- 대한약학회
- Keywords
- lung carcinogenesis; urethane; arsenic trioxide; cell cycle related proteins
- Citation
- 약 학 회 지, v.50, no.2, pp 84 - 92
- Pages
- 9
- Journal Title
- 약 학 회 지
- Volume
- 50
- Number
- 2
- Start Page
- 84
- End Page
- 92
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/29168
- ISSN
- 0377-9556
- Abstract
- The present study investigated an effect of arsenic trioxide on the urethane-induced lung carcinogenesis in
mice. To understand its carcinogenesis, we examined proliferating cell nuclear antigen (PCNA), apoptotic index as well as
cell cycle-related proteins (cyclin D1, p21, and p27). Urethane was injected intraperitoneally in ICR mice, and then they
were sacrificed at 5, 15, or 25 weeks following treatment of arsenic trioxide. Arsenic trioxide was given with tap water at
a concentration of 1 mg/l (low-dose) and 5 mg/l (high-dose) for 25 weeks. During the carcinogenesis, sequential histological
changes from hyperplasia to adenomas, and ultimately to overt carcinomas were noted. The development of hyperplasias,
adenomas, and carcinomas in the lung were slightly increased by the treatment of low-dose arsenic trioxide. However, there
is no correlation between dose and tumor multiplicity. The administration of low-dose arsenic trioxide, significantly
increased the tumor size. The proliferative index observed on 5 weeks after significantly increased. Cyclin D1 and p21 protein,
cell cycle related proteins, were more significantly increased in hyperplasia and adenoma in low dose arsenic treated
group than urethane alone group. The p27 protein expression did not show any significantly changes with arsenic treated
or untreated group. Low dose exposure to arsenic trioxide resulted in increased expression of cyclin D1 and p21 protein.
The present results indicate that low-dose treatment of arsenic trioxide, but not high dose of it, partly modulate the cellular
proliferation, cyclin D1, and p21 protein expression, and that this effect may contribute to accelerated development of lung
adenocarcinomas in urethane-induced mice.
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