성장인자 고정화를 위한 아질산분해 키토산 올리고머의 제조와 세포적합성Preparation and Cytocompatibility of Chitosan Oligomer by
Nitrous acid depolymerisation for Growth Factor Immobilization
- Authors
- 안세진; 손태일; 강학수; 이영기; 김구연; 김환범; 서윤정; 박보기; 최명호
- Issue Date
- 2006
- Publisher
- 한국키틴키토산학회
- Keywords
- chitosan oligomer; hydrolysis; cytocompatibility; GPC analysis; growth factor immobilization.; chitosan oligomer; hydrolysis; cytocompatibility; GPC analysis; growth factor immobilization.
- Citation
- Journal of Chitin and Chitosan, v.11, no.1, pp 42 - 46
- Pages
- 5
- Journal Title
- Journal of Chitin and Chitosan
- Volume
- 11
- Number
- 1
- Start Page
- 42
- End Page
- 46
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/29227
- ISSN
- 1229-4160
- Abstract
- Growth factors can be stabilized by immobilization with carbohydrates from thermal and proteolytic degradations. In thisstudy, we prepared chitosan oligomer which is suitable to immobilize growth factors and performed their cytocompatibility test.Chitosan oligomer is successfully prepared by the nitrous acid depolymerisation method and fractionated to each fraction havingdifferent molecular weights by the methanol precipitation method(Fraction A,B,C,D). The chemical structure of preparedchitosan oligomer is identified by IR and 1H-NMR analysis and the molecular weights of each fraction were determined by GPCanalysis. From the IR and 1H-NMR analysis, we identified that chitosan oligomer has the fundamental structure of chitosanpolymer after the nitrous acid depolymerisation. From the GPC analysis, the weight-average molecular weights of Fraction A, B,C, and D were determined as 8,400, 4,400, 3,200 and 1,200, respectively. By the results of cytocompatibility test, fraction Ashowed the higher survival ratio of NHDF cell line than other fractions and in lower concentration than 1 mg/mL, no toxicitieswere showed. In the result, it was considered that chitosan oligomer fraction A is suitable to immobilize growth factors.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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