닭 배자에서 Bisphenol A가 척수의 세포고사에 미치는 영향

Abstract

Objective : To investigate the effects of bisphenol A (BPA) on cell death pattern in neuronal development of chick embryos. Materials and Methods : We planned to compare the cytokinetic features in the normal chick embryo and those with BPA. Fifteen eggs were divided into three groups: the control group, BPA 50 µg/g egg group and BPA 200 µg/g egg group. Embryos were incubated for 56 hours (Hamburger & Hamilton stage 16) and then we injected BPA into embryos. The embryos were sectioned by 3 µm thickness at the level of wing buds and stained at 72 hours after incubation (HH stage 18). We observed cell death in the spinal cord using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method. Results : The TUNEL-positivity markedly increased in proportion to the doses of BPA. The number of TUNEL-positive cells per section was 15.2±2.14 in the control group, 34.6±3.44 in the BPA 50µg/g egg group, 87.6±4.32 in the BPA 200 g/g egg group. Furthermore the contour of spinal cord was deformed as the doses of BPA raised. Conclusion : BPA causes neuronal cell death and exerts cytotoxic effect on early chick embryos. It suggests that BPA might have an effect on cytogenesis during neural tube development.