An evolutionarily conserved non-synonymous SNP in a leucine-rich repeat domain determines anthracnose resistance in watermelon
- Authors
- Jang, Yoon Jeong; Seo, Minseok; Hersh, Craig P.; Rhee, Sun-Ju; Kim, Yongjae; Lee, Gung Pyo
- Issue Date
- Feb-2019
- Publisher
- Springer Verlag
- Citation
- Theoretical and Applied Genetics, v.132, no.2, pp 473 - 488
- Pages
- 16
- Journal Title
- Theoretical and Applied Genetics
- Volume
- 132
- Number
- 2
- Start Page
- 473
- End Page
- 488
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3240
- DOI
- 10.1007/s00122-018-3235-y
- ISSN
- 0040-5752
1432-2242
- Abstract
- Key message: A non-synonymous SNP of CC–NBS–LRR was firstly mapped to confer resistance to anthracnose in watermelon. Newly proposed LRR domain harboring the SNP is evolutionary conserved in the Cucurbitaceae and Fabaceae. Abstract: Anthracnose disease caused by Colletotrichum devastates many plants. Despite the importance of the disease, the mechanisms of resistance against it are poorly understood. Here, we identified a non-synonymous single-nucleotide polymorphism (SNP) located in a leucine-rich repeat domain as a marker for resistance to anthracnose race 1 in watermelon, using a combination of genetic analyses. We validated this SNP in segregating populations and 59 watermelon accessions using high-resolution melting assays and Sanger sequencing. We demonstrated that the resulting arginine-to-lysine substitution is particularly conserved among the Cucurbitaceae and Fabaceae. We identified a conserved motif, IxxLPxSxxxLYNLQTLxL, found in 1007 orthologues/paralogues from 89 plant species, and discovered that residue 18 of this motif could determine resistance to disease caused by external invaders. This study provides a step forward in understanding anthracnose resistance in watermelon, as well as functional and evolutionary insight into leucine-rich repeat proteins. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
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