키누레닌 대사산물에 의한 신경세포 손상에 대한 Magnolol의 보호효과에 대한 연구Magnolol Attenuates Neuronal Cell Death Induced by Kynurenine Metabolite
- Authors
- Lee, Chang Uk; Lee, Hyun Jung; Kim, Do Hee; Jang, Yeong Mi; Lee, Sang Hyung; Jeong, Yoonh Wa; Kim, Dae Jin; Chung, Yoon Hee; Kim, Kyung Yong; Kim, Sung Su; Lee, Won Bok
- Issue Date
- 2009
- Publisher
- 한국약용작물학회
- Keywords
- Magnolol; 3-hydroxykynureinine; Mitochondria; ROS; Caspase-3
- Citation
- 한국약용작물학회지, v.17, no.2, pp 145 - 150
- Pages
- 6
- Journal Title
- 한국약용작물학회지
- Volume
- 17
- Number
- 2
- Start Page
- 145
- End Page
- 150
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/32462
- ISSN
- 1225-9306
- Abstract
- This study investigated the protective roles and mechanism of magnolol, from the stem bark of Magnolia officinalis against potential neurotoxin 3-hydroxykynurenine (3-HK)-induced neuronal cell death. For the evaluation of protective role of magnolol, we examined cell viability, apoptotic nuclei, change of mitochondrial membrane potential and caspase activity in human neuroblastoma SH-SY5Y cells. It was found that 3-HK induces neuronal cell death in the human neuroblastoma SH-SY5Y cell line. The reduced cell viability produced characteristic features such as cell shrinkages, plasma membrane blebbing, chromatin condensation, and nuclear fragmentation. The cells treated with 3-HK showed an increase in the concentration of reactive oxygen species (ROS) as well as in caspase activity. In addition, both are involved in the 3-HK-induced apoptosis. Magnolol attenuated the cell viability reduction by 3-HK in both a dose- and time-dependent manner. Optical microscopy showed that magnolol inhibited the cell morphological features in the 3-HK-treated cells. Furthermore, the increase in the ROS concentration and the caspase activities by 3-HK were also attenuated by magnolol. These results showed that magnolol has a protective effect on the 3-HK induced cell death by inhibiting ROS production and caspase activity.
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