Chryseobacterium aureum sp. nov., isolated from the Han River, Republic of Korea

Abstract

A Gram-stain-negative, yellow-pigmented, non-motile, non-spore-forming, aerobic and rod-shaped bacterial strain, designated 17S1E7(T), was isolated from the Han River, Republic of Korea, and characterized by polyphasic taxonomy analyses. Strain 17S1E7(T) grew optimally on tryptic soy agar at 37 degrees C and pH 7.0 in the absence of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 17S1E7(T) belonged to the genus Chryseobacterium and was most closely related to Chryseobacterium culicis DSM 23031(T) (98.54 %). The average nucleotide identity value of strain 17S1E7(T) was 91.1 % to Chryseobacterium culicis DSM 23031(T), which was lower than the cut-off of 95-96%. The DNA G+C content of strain 17S1E7(T) was 37.4 mol%. Flexirubin-type pigments were produced. The predominant respiratory quinone was menaquinone 6. The major fatty acids of strain 17S1E7(T) were iso-C-15:0, summed feature 9 (iso-C-17:1 omega 9c and/or C-16:0 10-methyl), iso-C-17:0 3-OH and summed feature 3 (iso-C-15:0 2-OH and/or C-16:1 omega 7c). The predominant polar lipid was phosphatidylethanolamine. Based on polyphasic taxonomy data, strain 17S1E7(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aureum sp. nov. is proposed. The type strain is 17S1E7(T) (=KACC 19920(T)=JCM 33165(T)).