Lactobacillus plantarum CAU1055 ameliorates inflammation in lipopolysaccharide-induced RAW264.7 cells and a dextran sulfate sodium–induced colitis animal model
- Authors
- Choi, Sun-Hae; Lee, Sun-Hee; Kim, Min Gon; Lee, Hong Jin; Kim, Geun-Bae
- Issue Date
- Aug-2019
- Publisher
- Elsevier Inc.
- Keywords
- anti-inflammatory effect; dextran sulfate sodium; lactic acid bacteria; peptidoglycan; RAW264.7 cells
- Citation
- Journal of Dairy Science, v.102, no.8, pp 6718 - 6725
- Pages
- 8
- Journal Title
- Journal of Dairy Science
- Volume
- 102
- Number
- 8
- Start Page
- 6718
- End Page
- 6725
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33145
- DOI
- 10.3168/jds.2018-16197
- ISSN
- 0022-0302
1525-3198
- Abstract
- This study aimed to screen lactic acid bacteria (LAB) for their anti-inflammatory activity by using RAW264.7 cells and dextran sulfate sodium (DSS)-induced colitis. In all, 192 LAB strains were isolated from healthy human feces, of which 8 strains showed excellent nitric oxide (NO) inhibitory activity. Peptidoglycan extracts of these 8 LAB strains were subjected to NO assay, Western blot, and ELISA. Among the 8 tested strains, extracts of 4 strains significantly inhibited the production of NO, related enzyme activities such as inducible nitric oxide synthase and cyclooxygenase 2, and key cytokines such as tumor necrosis factor-α and IL-6 in RAW264.7 cells. The 4 strains belonged to Lactobacillus (CAU1054, CAU1055, CAU1064, and CAU1301). Oral administration of the 4 strains inhibited DSS-induced body weight loss, colon shortening, and colon damage in ICR mice. The colon tissue of the mice treated with Lactobacillus plantarum strain CAU1055 had significantly reduced levels of inducible nitric oxide synthase, cyclooxygenase 2, tumor necrosis factor-α, and IL-6. We found that strain CAU1055 could be used as a candidate probiotic strain for the prevention and treatment of inflammatory bowel disease. Further studies are warranted to confirm the mechanisms of interaction between peptidoglycan of L. plantarum strain CAU1055 and upstream cellular signaling mediators. © 2019 American Dairy Science Association
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33145)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.