극 저주파 전자기장이 N-bis(2-hydroxypropyl)nitrosamine과 Sulfadimethoxine에 의해 유발된 갑상샘 암에 미치는 영향Effects of Extremely Low Frequency Elecromagnetic Fields on Thyroid Carcinogenesis Induced by N-bis(2-hydroxypropyl)nitrosamine and Sulfadimethoxine
- Authors
- 이수환; 박용진; 박언섭; 김용석; 최유신; 김범규; 박성준; 정세민
- Issue Date
- 2009
- Publisher
- 대한외과학회
- Keywords
- Extremely low frequency magnetic field; Thyroid carcinogenesis; N-bis(2-hydroxypropyl)nitrosamine (DHPN); Apoptosis; Cell cycle-related proteins; 극 저주파 전자기장; 갑상샘 발암; 세포자멸사; 세포주기관련 단백
- Citation
- 대한외과학회지, v.77, no.3, pp 161 - 169
- Pages
- 9
- Journal Title
- 대한외과학회지
- Volume
- 77
- Number
- 3
- Start Page
- 161
- End Page
- 169
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33225
- ISSN
- 2288-6575
2288--679
- Abstract
- Purpose: Long-term exposure to extremely low-frequency (60 Hz) electromagnetic fields (ELF-EMF) raises the questions of the induction of biological effects including tumorigenesis. One mechanism through which ELF-MFS could influence neoplastic development is the imbalance of cellular proliferation and cell apoptosis. The present study investigated the effect of ELF-EMF on chemically-induced thyroid carcinogenesis in a rat.
Methods: We examined cellular proliferation index measured by anti-Ki-67 antigen, apoptosis, apoptosis related proteins such as caspase 3 and p53, and cell cycle-related proteins (cyclin D1 and p21WAF1/Cip1). Forty Male F344 rats received a subcutaneous N-bis(2-hydroxypropyl)nitrosamine (DHPN, 2,800 mg/kg) injection, and 1 week later were allowed free access to drinking water containing sulfadimethoxine (0.1%) for 12 weeks. Twenty rats were exposed by ELF-EMF. During the carcinogenesis, sequential histological changes from hyperplasia, adenoma, and ultimately to overt carcinomas were noted.
Results: The exposure group of ELF-EMF, significantly increases the number size of carcinomas. Also, the proliferative and apoptotic indices were significantly increased in the ELF-EMF exposure group than in the control group. The caspase 3 protein expression did not show any significant changes between ELF-EMF group and control group. The p53 protein was not detected in both ELF-EMF exposure and control group. Among the cell cycle related proteins, cyclin D1, not p21WAF1/Cip1, was significantly increased in adenomas and carcinomas in ELF-EMF exposure group compared with the control group.
Conclusion: Exposure of ELF-EMF effects on chemically-induced rat thyroid carcinogenesis as results of altered increase of cellular proliferation, apoptosis, and cyclin D1 expression.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
- College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33225)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.