3T3-L1 세포에서 지방세포형성 유도조절자 및 억제조절자의 발현에 대한 platycodin D의 효과Effects of Platycodin D on Gene Expressions of Pro-adipogenic and Anti-adipogenic Regulators in 3T3-L1 Cells
- Authors
- 이해용; 강련화; 조수현; 김성수; 김영식; 윤유식
- Issue Date
- Dec-2009
- Publisher
- 한국생명과학회
- Keywords
- Adipogenesis; platycodin D; 3T3-L1; KROX20; KLF15; CHOP
- Citation
- 생명과학회지, v.19, no.12, pp 1801 - 1807
- Pages
- 7
- Journal Title
- 생명과학회지
- Volume
- 19
- Number
- 12
- Start Page
- 1801
- End Page
- 1807
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33622
- ISSN
- 1225-9918
2287-3406
- Abstract
- Platycodin D, a major component of Platycodi radix, is known to have various activities including anti-inflammatory, anti-hyperlipidemic, anti-tumor activities and others. Recently, it was reported that platycodin D inhibits fat accumulation and adipogenesis. The aim of this study was to investigate whether various adipogenic regulators are modulated by platycodin D treatment during the adipogenesis of 3T3-L1 cells. mRNA levels of terminal markers of adipogenesis such as ADIPOQ (adiponectin) and GLUT (glucose transporter) 4, which were quantified by real time PCR, were decreased by platycodin D treatment. mRNA expression of PPAR (peroxisome proliferator-activated receptor) γ and C/EBP (CCAAT/enhaner binding protein) α, which are central transcription factors of adipogenesis, were also decreased by platycodin D treatment. To elucidate the detailed molecular mechanism of platycodin D-induced inhibition of adipogenesis, we analyzed mRNA expression of upstream regulators of PPARγ and C/EPBα. mRNA levels of the pro-adipogenic regulators, KROX20 and KLF (Kruppel-like factor) 15 were markedly down-regulated by platycodin D treatment. On the other hand, mRNA expression of CHOP (C/EBP homologous protein), an anti-adipogenic regulator, was significantly up-regulated by platycodin D treatment. mRNA levels of other pro-adipogenic regulators, C/EBPβ and C/EPBδ, were not affected by platycodin D treatment, and another anti-adipogenic regulator, C/EBPγ was also not affected by platycodin D treatment. Taken together, these results suggest that platycodin D-induced inhibition of adipogenesis is mediated by gene interactions including the down-regulation of pro-adipogenic regulators, KROX20 and KLF15, and the up-regulation of an anti-adipogenic regulator, CHOP.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33622)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.