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HPLC를 이용한 혼합차의 Hesperidin 정량Determination of hesperidin in mixed tea by HPLC
- Issue Date
- Jun-2011
- Publisher
- 충남대학교 농업과학연구소
- Keywords
- Hesperidin; Mixed tea; Quantitative analysis; HPLC
- Citation
- Korean Journal of Agricultural Science, v.38, no.2, pp 295 - 299
- Pages
- 5
- Journal Title
- Korean Journal of Agricultural Science
- Volume
- 38
- Number
- 2
- Start Page
- 295
- End Page
- 299
- ISSN
- 2466-2402
2466-2410
- Abstract
- The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (water:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 nm). The flow rate was kept constant at 1.0 ml/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 mg/g, respectively).
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Collections - College of Biotechnology & Natural Resource > ETC > 1. Journal Articles
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