Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection
- Authors
- Lee, Taek; Kim, Ga Hyeon; Kim, Soo Min; Hong, Keonyoung; Kim, Younghun; Park, Chulhwan; Sohn, Hiesang; Min, Junhong
- Issue Date
- Oct-2019
- Publisher
- Elsevier B.V.
- Keywords
- AI virus detection; Biosensor; HA protein; Hollow Au spike-like nanoparticles; Localized surface plasmon resonance; Multi-functional DNA
- Citation
- Colloids and Surfaces B: Biointerfaces, v.182
- Journal Title
- Colloids and Surfaces B: Biointerfaces
- Volume
- 182
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/34084
- DOI
- 10.1016/j.colsurfb.2019.06.070
- ISSN
- 0927-7765
1873-4367
- Abstract
- In the present study, we fabricated a label-free avian influenza (AIV H5N1) detection biosensor composed of a multi-functional DNA 3 way-Junction (3 W J) on a hollow Au spike-like nanoparticle (hAuSN) using a localized surface plasmon resonance (LSPR) method. To construct the multi-functional DNA (MF-DNA) as a bioprobe, the 3 W J was introduced. The proposed AIV detection bioprobe should contain three functionalities: target recognition, signal amplification, and connection to substrate. To achieve this goal, each piece of the DNA 3 W J was tailored to a hemagglutinin (HA) binding aptamer, FAM dye and thiol group, respectively. The assembly of each DNA 3 W J functional fragment was then confirmed by TBM-Native PAGE. Moreover, the hAuSN was immobilized on the indium-tin-oxide (ITO) substrate for LSPR measurement. The DNA 3 W J was immobilized onto the hAuSN electrode through the thiol-group of DNA 3 W J. The fabricated DNA 3 W J/hAuSN heterolayer on the ITO substrate was investigated by field emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). LSPR experiments were conducted to confirm HA protein binding to the DNA 3 W J/ hAuSN -modified electrode. The proposed biosensor can detected the HA protein in PBS buffer (LOD: 1 pM) as well as in the diluted chicken serum (LOD: 1 pM). The present study details a label-free, simple fabrication method consisted of DNA 3 W J/ hAuSN heterolayer that uses easy-to-tailor elements to detect not only AIV but also various viruses detection platform easily. © 2019 Elsevier B.V.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of ICT Engineering > School of Integrative Engineering > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/34084)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.