Electrochemical peptide sensor for diagnosing adenoma-carcinoma transition in colon cancer
- Authors
- Lim, Jong Min; Ryu, Myung Yi; Yun, Jong Won; Park, Tae Jung; Park, Jong Pil
- Issue Date
- Dec-2017
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Colon cancer; LRG1; Affinity peptide; Sensitivity; Electrochemical sensor
- Citation
- BIOSENSORS & BIOELECTRONICS, v.98, pp 330 - 337
- Pages
- 8
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 98
- Start Page
- 330
- End Page
- 337
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3493
- DOI
- 10.1016/j.bios.2017.07.013
- ISSN
- 0956-5663
1873-4235
- Abstract
- Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths. Therefore, more sensitive and early diagnostic methods for CRC are urgently needed. In this study, an efficient electrochemical biosensor for early diagnosis of adenoma-to-carcinoma progression that employs a series of chemically modified affinity peptides was developed. A series of amino acid-substituted and cysteine-incorporated synthetic peptides with flexible linkers was chemically synthesized and immobilized to a gold sensor layer; performance of the sensor was monitored using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides (LRG1 BP1-BP4) specific for the LRG1 biomarker as a target protein were chosen according to a quantitative current decrease and dynamic impedance increase by CV and EIS, respectively. Using EIS, the Kd value of the LRG1 BP3 peptide was found to be 8.3 +/- 2.7 nM. The applicability of the sensor to detect LRG1 proteins was confirmed in human plasma from colorectal adenomas and carcinomas (n = 20 in each group). The detection of LRG1 in accordance with the ARC, value (electron-transfer resistance at the electrode surface) of the sensor layer incorporating LRG1 BP3 peptides showed a statistically significant difference (p < 0.001) between adenomas and carcinomas, indicating that the potential use of this biosensing platform for detecting the CRC biomarker, as well as for monitoring the colorectal adenoma-to-carcinoma transition in an electrochemically miniaturized biosensor (e-chem biosensor) in point-of-care testing, is possible.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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