흰민들레(Taraxacum coreanum) 활성획분인 Ethylacetate Fraction의 Peroxynitrite에 대한 산화적 스트레스 개선 효과Protective Effects of Ethylacetate Fraction from Taraxacum coreanum against Peroxynitrite-induced Oxidative Damage under Cellular System
- Authors
- 최지명; 최미진; 이설림; 야마베노리꼬; 이상현; 조은주
- Issue Date
- 2012
- Publisher
- 대한암예방학회
- Keywords
- Taraxacum coreanum; LLC-PK1; Peroxynitrite; Superoxide anion; Nitric oxide
- Citation
- 대한암예방학회지, v.17, no.3, pp 251 - 256
- Pages
- 6
- Journal Title
- 대한암예방학회지
- Volume
- 17
- Number
- 3
- Start Page
- 251
- End Page
- 256
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/35366
- ISSN
- 2288-3649
2288-3657
- Abstract
- The EtOAc fraction from Taraxacum coreanum showed the strongest radical scavenging activity. The protective activity of active EtOAc fraction against the oxidative stress was investigated under cellular system using LLC-PK1 renal epithelial cell. The LLC-PK1 cells showed loss of the cell viability and elevation for lipid peroxidation by the treatment with generator of peroxynitrite (ONOO-), 3-mor- -pholinosydnonimine, and its precursors, nitric oxide (NO) and superoxide anion (O2 ), produced by sodium nitroprusside and pyrogallol, respectively. However, the treatment of the EtOAc fraction significantly, dose-dependently, recovered cell viability and inhibited lipid peroxidation against both ONOO- itself, and its precursors of NO and O2 . At 100μg/ml of the EtOAC fraction, the cell viability was significantly increased to 93.4% from 61.0%, and lipid peroxidation was inhibited to 0.104 nmol/mg protein from 0.494 nmol/mg protein. The present study suggests that T. coreanum would have the protective potential from ONOO--induced oxidative stress.
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