유근피(楡根皮)에 존재하는 matrix metalloproteinase-9 억제 물질의 분리 및 정제
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한기정 | - |
dc.contributor.author | 이광수 | - |
dc.contributor.author | 공광훈 | - |
dc.contributor.author | 조성희 | - |
dc.date.available | 2019-10-14T00:40:07Z | - |
dc.date.issued | 2003-06 | - |
dc.identifier.issn | 1225-0163 | - |
dc.identifier.issn | 2288-8985 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/36797 | - |
dc.description.abstract | 극성이 다른 여러 가지 용매를 사용하여 느릅나무의 뿌리 껍질(이하 유근피)을 추출하였다. MeOH 추출물이 SK-Hep-1에서 분리된 matrix metalloproteinase-9(MMP-9)의 zymography에서 저해능을 나타내었다. 분리 정제한 물질의 분자량은 GC-MS spectrum에서 M+=281인 것으로 나타났고 MMP-9의 활성은 314.7 ㎍/g에서 47% 억제되는 것으로 나타났다. 그리고 SK-Hep-1 세포주는 31.47 ㎍/g에서 60%의 생존율을 나타났다. | - |
dc.description.abstract | Root bark of Ulmus davidiana Plancn. var. japonica Nakai was extracted by using several solvents with different polarities. Each extract was treated on the MMPs obtained from SK-Hep-1 in order to investigate inhibition effect. Zymography of MMPs showed that MeOH extract has significant inhibition effect. On the GC-MS analysis the highest mass to charge ratio (m/z) of the purified substance was 281. Also, on zymography of MMPs the substance showed 47% inhibition effect at the concentration of 314.7 ㎍/g. Cell viability of SK-Hep-1 was 60% at 31.47 ㎍/g. | - |
dc.format.extent | 6 | - |
dc.publisher | 한국분석과학회 | - |
dc.title | 유근피(楡根皮)에 존재하는 matrix metalloproteinase-9 억제 물질의 분리 및 정제 | - |
dc.title.alternative | Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. Var. japonica Nkai. | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 분석과학, v.16, no.3, pp 179 - 184 | - |
dc.identifier.kciid | ART001195551 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 184 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 179 | - |
dc.citation.title | 분석과학 | - |
dc.citation.volume | 16 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Zymography | - |
dc.subject.keywordAuthor | MMPs | - |
dc.subject.keywordAuthor | GC-MS | - |
dc.description.journalRegisteredClass | kciCandi | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.