유근피(楡根皮)에 존재하는 matrix metalloproteinase-9 억제 물질의 분리 및 정제Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. Var. japonica Nkai.
- Authors
- 한기정; 이광수; 공광훈; 조성희
- Issue Date
- Jun-2003
- Publisher
- 한국분석과학회
- Keywords
- Zymography; MMPs; GC-MS
- Citation
- 분석과학, v.16, no.3, pp 179 - 184
- Pages
- 6
- Journal Title
- 분석과학
- Volume
- 16
- Number
- 3
- Start Page
- 179
- End Page
- 184
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/36797
- ISSN
- 1225-0163
2288-8985
- Abstract
- 극성이 다른 여러 가지 용매를 사용하여 느릅나무의 뿌리 껍질(이하 유근피)을 추출하였다. MeOH 추출물이 SK-Hep-1에서 분리된 matrix metalloproteinase-9(MMP-9)의 zymography에서 저해능을 나타내었다. 분리 정제한 물질의 분자량은 GC-MS spectrum에서 M+=281인 것으로 나타났고 MMP-9의 활성은 314.7 ㎍/g에서 47% 억제되는 것으로 나타났다. 그리고 SK-Hep-1 세포주는 31.47 ㎍/g에서 60%의 생존율을 나타났다.
Root bark of Ulmus davidiana Plancn. var. japonica Nakai was extracted by using several solvents with different polarities. Each extract was treated on the MMPs obtained from SK-Hep-1 in order to investigate inhibition effect. Zymography of MMPs showed that MeOH extract has significant inhibition effect. On the GC-MS analysis the highest mass to charge ratio (m/z) of the purified substance was 281. Also, on zymography of MMPs the substance showed 47% inhibition effect at the concentration of 314.7 ㎍/g. Cell viability of SK-Hep-1 was 60% at 31.47 ㎍/g.
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