Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins
- Authors
- Kwon, J.-H.; Kim, H.-H.; Cho, H.-B.; Cha, Y.J.; Lee, J.
- Issue Date
- Oct-2019
- Publisher
- Royal Society of Chemistry
- Citation
- Nanoscale, v.11, no.39, pp 18282 - 18289
- Pages
- 8
- Journal Title
- Nanoscale
- Volume
- 11
- Number
- 39
- Start Page
- 18282
- End Page
- 18289
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/37883
- DOI
- 10.1039/c9nr06160f
- ISSN
- 2040-3364
2040-3372
- Abstract
- The aim of this study is to develop a novel method for the accurate diagnosis of the infection status of viral diseases, which requires discriminated and quantitative detection of different anti-virus immunoglubulin subtypes. Considering hepatitis A as a representative model disease, viral antigen nanoparticles (vAgNPs) were designed and synthesized by genetically presenting hepatitis A virus (HAV) antigens on the surface of human heavy chain ferritin (hFTH) nanoparticles to detect anti-HAV antibodies with discriminating immunoglobulin subtypes M and G (IgM and IgG, respectively). The vAgNPs also display multi-copies of hexa-histidine peptide (H6) on their surface to chemisorb gold ions (Au3+), which is vital for the autonomous generation of quantitatively meaningful detection signals. The quantitative level of anti-HAV IgM or IgG in 30 patient sera was successfully analyzed using the vAgNPs of HAV, which was performed through label-free one-step-immunoassay based on the self-enhancement of optical signals from gold nanoparticles clustered on the viral antigen nanoparticles. The diagnostic performance was compared with that of enzyme-linked immunosorbent assay (ELISA), which did not enable accurate quantitative assay due to the poor linearity between the antibody concentration and detection signal. Furthermore, these vAgNP-based immunoassays did not produce any false negative/positive signals, indicating 100% sensitivity and 100% specificity. © 2019 The Royal Society of Chemistry.
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