Identification and biosynthesis of C-24 ethylidene brassinosteroids in Arabidopsis thaliana
- Authors
- Roh, Jeehee; Yeom, Hyeon Soong; Jang, Hwanhee; Kim, Sunyoung; Youn, Ji Hyun; Kim, Seong-Ki
- Issue Date
- Oct-2017
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Arabidopsis thaliana; Biosynthesis; Brassinosteroids; CYP85As; Homodolichosterone
- Citation
- JOURNAL OF PLANT BIOLOGY, v.60, no.5, pp 533 - 538
- Pages
- 6
- Journal Title
- JOURNAL OF PLANT BIOLOGY
- Volume
- 60
- Number
- 5
- Start Page
- 533
- End Page
- 538
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3841
- DOI
- 10.1007/s12374-017-0132-x
- ISSN
- 1226-9239
1867-0725
- Abstract
- Isofucosterol is a major 4-demethylsterol which has an ethylidene group at C-24 in Arabidopsis thaliana. To evaluate the presence of brassinosteroids (BRs) with the same carbon skeleton as that of isofucosterol, a large quantity of A. thaliana was extracted and purified. GC-MS/selected ion monitoring analysis verified that 6-deoxohomodolichosterone and homodolichosterone are present in Arabidopsis. An enzyme solution prepared from wild type Arabidopsis successfully mediated conversion of 6-deoxohomodolichosterone to homodolichosterone. However, a double mutant cyp85a1/cyp85a2 could not catalyze the conversion, implying that in A. thaliana the C-6 oxidation of 6-deoxohomodolichosterone to homodolichosterone seems to be catalyzed by CYP85A1 and/or CYP85A2. In yeast, both heterologously expressed CYP85A1 and CYP85A2 catalyzed the C-6 oxidation of 6- deoxohomodolichosterone to homodolichosterone, but the conversion rate in CYP85A2/V60/WAT21 was significantly higher than that in CYP85A1/V60/WAT21, indicating that C-6 oxidation of 6-deoxohomodolichosterone to homodolichosterone is mainly catalyzed by CYP85A2 in A. thaliana. Taken together, this study strongly suggests that a biosynthetic pathway for the production of 6-deoxohomodolichosterone and homodolichosterone is functional, and CYP85As have important roles in 24-ethylidene biosynthesis in A. thaliana.
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