국가 바이오모니터링 프로그램을 위한 소변 중 금속류 동시분석법 개발 및 검증Development and Validation of the Simultaneous Analytical Method of Urinary Metals and Metalloids for the National Biomonitoring Programs
- Authors
- 조용민; 양민호; 임호섭; 차상원; 이재익; 김기훈; 한상범
- Issue Date
- Dec-2019
- Publisher
- 한국환경보건학회
- Keywords
- Urinary metals; ICP-MS; simultaneous analysis; biomonitoring
- Citation
- 한국환경보건학회지, v.45, no.6, pp 594 - 604
- Pages
- 11
- Journal Title
- 한국환경보건학회지
- Volume
- 45
- Number
- 6
- Start Page
- 594
- End Page
- 604
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38427
- DOI
- 10.5668/JEHS.2019.45.6.594
- ISSN
- 1738-4087
- Abstract
- Objectives: This study developed and validated an analysis method of urinary metals and metalloids that can be applied inductively with coupled plasma mass spectrometry (ICP-MS).
Methods: 0.3 mL of urine was used to analyze 25 metal and metalloid compounds using ICP-MS. The validation of the analytical method included linearity, accuracy, precision, and the calculation of detection limits.
In addition, a comparison test was performed with the graphite furnace atomic absorption spectrometry (GF-AAS) method, which is the current standard method, with urine samples of 66 healthy subjects.
Results: The linearities (R2 ) of calibration curves of all 25 compounds were ≥ 0.999. Of the 25 compounds, the intra-day and inter-day accuracy% of 17 and 20 met ≤15%, respectively. In addition, fifteen compounds showed ≤15% recovery% for certificated reference materials. Intraclass correlation coefficients of the comparison between the current methods and new methods in this study were 0.952 (p-value<0.001) and 0.911 (p-value<0.001) for urinary cadmium and mercury, respectively.
Conclusion: This study proposes an efficient simultaneous methodology that can analyze multi elements in smaller sample amounts. More reproduction experiments are needed in the future.
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