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Description of Mycobacterium chelonae subsp bovis subsp nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp chelonae subsp nov.

Authors
Kim, Byoung-JunKim, Ga-NaKim, Bo -RamJeon, Che OkJeong, JosephLee, Seon HoLim, Ji-HunLee, Seung-HeonKim, Chang KiKook, Yoon-HohKim, Bum-Joon
Issue Date
Oct-2017
Publisher
MICROBIOLOGY SOC
Keywords
rapid growing mycobacteria; multilocus sequence typing; Mycobacterium chelonae; Mycobacterium chelonae subsp chelonae; Mycobacterium chelonae subsp bovis.
Citation
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v.67, no.10, pp 3882 - 3887
Pages
6
Journal Title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volume
67
Number
10
Start Page
3882
End Page
3887
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3851
DOI
10.1099/ijsem.0.002217
ISSN
1466-5026
1466-5034
Abstract
Three rapidly growing mycobacterial strains, QIA-37 T, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752 T by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7% sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae. DNA-DNA hybridization values of 78.2% between QIA-37 T and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae. Phylogenetic analysis based on whole-genome sequences revealed a 95.44 +/- 0.06% average nucleotide identity (ANI) value with M. chelonae, slightly higher than the 95.0% ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 degrees C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae. Therefore, we propose an emended description of Mycobacterium chelonae, and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752(T)(=CCUG 47445(T) = CIP 104535(T) = DSM 43804(T) = JCM 6388(T) = NCTC 946(T)) and QIA-37(T) (= KCTC 39630(T) = JCM 30986(T)) are the type strains of the two novel subspecies.
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자연과학대학 (생명과학과)
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