Expression and In Vitro Function of Anti-Breast Cancer Llama-Based Single Domain Antibody VHH Expressed in Tobacco Plantsopen access
- Authors
- Park, Se Ra; Lee, Jeong-Hwan; Kim, Kibum; Kim, Taek min; Lee, Seung Ho; Choo, Young-Kug; Kim, Kyung Soo; Ko, Kisung
- Issue Date
- Feb-2020
- Publisher
- NLM (Medline)
- Keywords
- breast cancer; HER2; Herceptin; plant antibody; VHH antibody
- Citation
- International journal of molecular sciences, v.21, no.4
- Journal Title
- International journal of molecular sciences
- Volume
- 21
- Number
- 4
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38539
- DOI
- 10.3390/ijms21041354
- ISSN
- 1661-6596
1422-0067
- Abstract
- Overexpression of human epidermal growth factor receptor type 2 (HER2) is considered as a prognostic factor of breast cancer, which is positively associated with recurrence when cancer metastasizes to the lymph nodes. Here, we expressed the single variable domain on a heavy chain (VHH) form of anti-HER2 camelid single domain antibody in tobacco plants and compared its in vitro anticancer activities with the anti-HER2 full size antibody. The gene expression cassette containing anti-HER2 camelid single domain antibody VHH fused to human IgG Fc region with KDEL endoplasmic reticulum (ER) (VHH-FcK) was transferred into the tobacco plant via the Agrobacterium-mediated transformation. The transformants were screened with polymerase chain reaction and Western blot analyses. Enzyme-linked immunosorbent assay (ELISA) confirmed the binding of the purified anti-HER2 VHH-FcK to the HER2-positive breast cancer cell line, SK-BR-3. Migration assay results confirmed anticancer activity of the plant-derived anticancer camelid single chain antibody. Taken together, we confirmed the possibility of using anti-HER2 VHH-FcK as a therapeutic anticancer agent, which can be expressed and assembled and purified from a plant expression system as an alternative antibody production system.
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