Butyrate attenuated fat gain through gut microbiota modulation in db/db mice following dapagliflozin treatmentopen access
- Authors
- Oh, Tae Jung; Sul , Woo Jun; Oh, Han Na; Lee, Yun-Kyung; Lim, Hye Li; Choi, Sung Hee; Park, Kyong Soo; Jang, Hak Chul
- Issue Date
- Dec-2019
- Publisher
- Nature Research
- Citation
- Scientific Reports, v.9, no.1
- Journal Title
- Scientific Reports
- Volume
- 9
- Number
- 1
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/39368
- DOI
- 10.1038/s41598-019-56684-5
- ISSN
- 2045-2322
- Abstract
- We investigated the effect of a combination treatment with dapagliflozin (Dapa), a sodium-glucose cotransporter-2 inhibitor and butyrate on weight change in db/db mice. Six-week-old male db/db mice were assigned to four groups: vehicle with normal chow diet (NCD), Dapa with NCD, vehicle with 5% sodium butyrate-supplemented NCD (NaB), or Dapa with 5% NaB. After six weeks of treatment, faecal microbiota composition was analysed by sequencing 16S ribosomal RNA genes. In the vehicle with NaB and Dapa + NaB groups, body weight increase was attenuated, and amount of food intake decreased compared with the vehicle with the NCD group. The Dapa + NaB group gained the least total and abdominal fat from baseline. Intestinal microbiota of this group was characterized by a decrease of the Firmicutes to Bacteroidetes ratio, a decrease of Adlercreutzia and Alistipes, as well as an increase of Streptococcus. In addition, the proportion of Adlercreutzia and Alistipes showed a positive correlation with total fat gain, whereas Streptococcus showed a negative correlation. Inferred metagenome function revealed that tryptophan metabolism was upregulated by NaB treatment. We demonstrated a synergistic effect of Dapa and NaB treatment on adiposity reduction, and this phenomenon might be related to intestinal microbiota alteration. © 2019, The Author(s).
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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