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An effective and rapid method for RNA preparation from non-conventional yeast speciesopen access
- Authors
- Lee, Dong Wook; Hong, Chang Pyo; Kang, Hyun Ah
- Issue Date
- Dec-2019
- Publisher
- Academic Press Inc.
- Keywords
- RNA extractionNon-saccharomyces yeastsCell wallHyphae
- Citation
- Analytical Biochemistry, v.586
- Journal Title
- Analytical Biochemistry
- Volume
- 586
- ISSN
- 0003-2697
1096-0309
- Abstract
- The increased use of high-throughput RNA-based analysis has spurred the demand for rapid and simple preparation of high quality RNA. RNA preparation from non-conventional yeasts having diverse cell wall and morphological characteristics is often inefficient using current methods adapted for the model yeast, Saccharomyces cerevisiae. We report a simple RNA preparation method based on glass bead-mediated breakage in a formamide/EDTA solution. High quality RNA is generated within 15 min from various non-conventional yeasts species. The obtained RNA can be directly used for experimentation without further RNA purification and buffer exchange. © 2019 The Authors
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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