돼지 로타바이러스(Gottfried 주)의 VP4 항원구조분석Analysis of antigenic sites on the VP4 of porcine rotavirus, Gottfried strain
- Authors
- 송윤경; 김원용; 강신영
- Issue Date
- Sep-2001
- Publisher
- 대한수의학회
- Keywords
- Rotavirus; VP4; antigenic site; N-MAb resistant escape mutant; SSCP
- Citation
- 대한수의학회지, v.41, no.3, pp 343 - 350
- Pages
- 8
- Journal Title
- 대한수의학회지
- Volume
- 41
- Number
- 3
- Start Page
- 343
- End Page
- 350
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/39810
- ISSN
- 2466-1384
2466-1392
- Abstract
- The neutralization epitopes of the outer capsid protein VP4 of a porcine rotavirus, Gottfried strain, were studied using neutralizing monocolonal antibodies(N-MAbs). Eight N-MAbs which are specific for the VP4 of Gottfried strain were used for analyzing the antigenic sites of VP4. Three different approaches were used for this analysis; i)testing the serological reactivity of each N-MAb against different G and P types of human and animal rotavirusese ii) analyzing N-MAb-resistant viral escape mutants and iii) performing nucleotide sequence analysis of the VP4 gene of each N-MAb-resistant viral escape mutant. From experimental results, at least four antigenic sites(I, II, III, and IV) were identified. Antigenic site I recognized by N-MAbs 24B9, 23G10, and 26A2 was separated from antigenic site II recognized by N-MAbs 30H5, 32B3, and 29B3. However, these antigenic sites were overlapped with antigenic site III recognized by N-MAb 21A1. The other antigenic site IV recognized by N-MAb 16D2 was separated from antigenic sites I, II, and III.
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