A float culture method for fungal secondary metabolism study using hydrophilic polyvinylidene fluoride membranes
- Authors
- Tian, Fei; Lee, Sang Yoo; Woo, So Young; Choi, Hwa Young; Chun, Hyang Sook
- Issue Date
- 15-Jun-2020
- Publisher
- Academic Press Inc.
- Keywords
- Aflatoxin; Colony morphology; Fungal incubation; Gene expression; Polyvinylidene fluoride membrane
- Citation
- Analytical Biochemistry, v.599
- Journal Title
- Analytical Biochemistry
- Volume
- 599
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/42755
- DOI
- 10.1016/j.ab.2020.113722
- ISSN
- 0003-2697
1096-0309
- Abstract
- Fungal metabolism is affected by both the developmental stage and cultivation conditions. Fungal growth in solid culture reflects natural conditions more closely than growth in liquid culture; however, because the mycelium cannot be harvested easily and the medium composition cannot be modified during incubation, the approach has some limitations when compared to liquid culture methods. The float culture incubation method introduced herein enables fungus to develop similar colonies to those on solid culture. This is a simple method that leads to the production of high-quality RNA samples. © 2020
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