Ruegeria sediminis sp. nov., isolated from tidal flat sediment

Abstract

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain, designated CAU 1488(T), was isolated from tidal flat sediment, and its taxonomic position was investigated using a polyphasic approach. The organism grew optimally at a temperature of 30 degrees C, at pH 7.0-7.5 and in the presence of 0-6% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1488(T) forms a lineage distinct from Ruegeria marisrubri ZGT 118(T) (97.9%), Ruegeria marina ZH17(T) (97.6%), Ruegeria lacuscaerulensis ITI 115(T) (97.5%), Ruegeria pomeroyi DSS-3 1 (97.1%), Ruegeria profundi ZGT108(T) (97.0%), Ruegeria intermedia CC-GIMAT-2(T) (96.8%), Ruegeria atlantica CECT 4292(T) (96.7%) and Ruegeria kandeliae J95(T) (95.9%). Genome sequencing revealed that CAU 1488(T )had a genome size of 4.23 Mbp and a G+C content of 63.2 mol%. Overall genome related indexes including average nucleotide identity and digital DNA-DNA hybridization values were 75.0-83.0% and 26.2%, which are below the cutoffs of 95 and 70%, respectively, indicating that strain CAU 1488(T) represents a distinct species from the members of the genus Ruegeria. The predominant quinone was ubiquinone-10 (Q-10). The major fatty acids were summed feature 8 (C-18:1 omega 7c/omega 6c; 60.7%) and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and unidentified aminolipids. On the basis of phenotypic, chemotaxonomic and genomic data, strain CAU 1488(T) constitutes a novel species of the genus Ruegeria, for which the name Ruegeria sediminis sp. nov. is proposed. The type strain is CAU 1488(T) (=KCTC 62996(T) =NBRC 113693(T)).