Surface-Enhanced Raman Scattering of Dopamine on Self-Assembled Gold Nanoparticles
- Authors
- An, Jeung-Hee; El-Said, Waleed Ahmed; Yea, Cheol-Heon; Kim, Tae-Hyung; Choi, Jeong-Woo
- Issue Date
- May-2011
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Surface-Enhanced Raman Spectroscopy; Dopamine; Gold Nanoparticles; Anti-Dopamine; Immunosensor
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.11, no.5, pp 4424 - 4429
- Pages
- 6
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 11
- Number
- 5
- Start Page
- 4424
- End Page
- 4429
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/43496
- DOI
- 10.1166/jnn.2011.3688
- ISSN
- 1533-4880
1533-4899
- Abstract
- Dopamine, a potent neurotransmitter in the brain, influences a variety of motivated behaviors and plays a major role in Parkinson's disease. In this study, the Raman signal of dopamine was detected on a fabricated nanoparticle-immobilized glass surface by surface-enhanced raman spectroscopy (SERS). Amine-modified glass was prepared by the self-assembly of amine-terminated silane on substrate, followed by the deposition of gold nanoparticles. The gold nanoparticles deposited on the glass surface were functionalized by anti-dopamine or dopamine. The antigen-dopamine was captured by antibody-assembled gold substrate and detected by SERS. The optical properties and morpology of the glass substrate with immobilized gold nanoparticles were analyzed by scanning electron microscopy and UV-VIS absorption spectroscopy. The Raman spectrum of dopamine displayed broad bands at 1267, 1331, 1158, 1478, 1578 and 1584 cm(-1). The strongest peaks in the spectra (at 1267 and 1478 cm(-1)) were identified as phenolic carbon-oxygen and phenyl C=C stretches, respectively. A working curve of the SERS signal constructed from cathecol ring vibration versus antigen-dopamine concentration was obtained at 1478 cm(-1), and the non-optimized detection limit for anti-dopamine surface antigen was as low as 1 ng/ml. These results suggest that SERS-based immunosensor can be a promising tool for the detection and screening of neurotransmitters.
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