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Nanoscale fabrication of a peptide layer in cell chip to detect effects of environmental toxins on HEK293 cells
- Issue Date
- Dec-2010
- Publisher
- SPRINGER
- Keywords
- Cyclic voltammetry; Environmental toxicants; HEK-293 cells; RGD peptide; Scanning tunneling microscopy; Surface plasmon resonance
- Citation
- BIOTECHNOLOGY LETTERS, v.32, no.12, pp 1797 - 1802
- Pages
- 6
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 32
- Number
- 12
- Start Page
- 1797
- End Page
- 1802
- ISSN
- 0141-5492
1573-6776
- Abstract
- A cysteine-terminated C(RGD)(4) peptide film was fabricated on a gold electrode for improving the attachment of cells. The electrochemical signals of cyclic voltammogram from cells on a C(RGD)(4) deposited electrode was enhanced from 0.27 to 0.49 mu A compared to a bare electrode. The developed cell-based sensor determined the effect of bisphenol-A (BPA) and dichlorodiphenyltrichloroethane (DTT) on the viability of HEK-293 cells by detecting decrease of reduction peaks (1.12-0.15 mu A for BPA and 0.81-0.29 mu A for DDT) after the treatment of environmental chemicals. This developed system can be a powerful tool for the monitoring of environmental toxicants.
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Collections - College of ICT Engineering > School of Integrative Engineering > 1. Journal Articles
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