Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays
- Authors
- Wu, Yixuan; Choi, Namhyun; Chen, Hao; Dang, Hajun; Chen, Lingxin; Choo, Jaebum
- Issue Date
- 4-Feb-2020
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.92, no.3, pp 2628 - 2634
- Pages
- 7
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 92
- Number
- 3
- Start Page
- 2628
- End Page
- 2634
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/44303
- DOI
- 10.1021/acs.analchem.9b04522
- ISSN
- 0003-2700
1520-6882
- Abstract
- We report a surface-enhanced Raman scattering (SERS)-based polymerase chain reaction (PCR) assay platform for the sensitive and rapid detection of a DNA marker (pagA) of Bacillus anthracis. Real-time quantitative PCR (RT-qPCR) has been recently considered a gold standard for the quantitative evaluation of a target gene, but it still suffers from the problem of a long thermocycling time. To address this issue, we developed a conceptually new SERS-PCR platform and evaluated its performance by sequentially measuring the Raman signals of B. anthracis DNA after the completion of different thermocycling numbers. According to our experimental data, SERS-PCR has lower limits of detection (LODs) than RT-qPCR under the small cycle number of 20. Particularly, it was impossible to detect a target DNA amplicon using RT-qPCR before the number of cycles reached 15, but SERS-PCR enabled DNA detection after only five cycles with an LOD value of 960 pM. In addition, the dynamic range for SERS-PCR (0.1-1000 pM) is wider than that for RTqPCR (150-1000 pM) under the same condition. We believe that this SERS-PCR technique has a strong potential to be a powerful tool for the rapid and sensitive diagnosis of infectious diseases in the near future.
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