Functional fusion proteins and prevention of electrode fouling for a sensitive electrochemical immunosensor
- Authors
- Kim, A-Ram; Park, Tae Jung; Kim, Minseok S.; Kim, In-Ho; Kim, Ki-Suk; Chung, Kwang Hoe; Ko, Sungho
- Issue Date
- May-2017
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Electrochemical immunosensor; Electrode fouling; Silica binding polypeptide; Protein G; Silica nanoparticle; Cyclic olefin copolymer
- Citation
- ANALYTICA CHIMICA ACTA, v.967, pp 70 - 77
- Pages
- 8
- Journal Title
- ANALYTICA CHIMICA ACTA
- Volume
- 967
- Start Page
- 70
- End Page
- 77
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4439
- DOI
- 10.1016/j.aca.2017.02.026
- ISSN
- 0003-2670
1873-4324
- Abstract
- A highly sensitive electrochemical immunosensor was developed by preventing electrode fouling and using a novel fusion protein of silica binding polypeptides (SBP)-protein G (ProG) created by recombinant DNA technology as a functional crosslinker for rapid and self-oriented immobilization of antibodies onto silica nanoparticles (SiNPs). Antibody immobilization onto the SiNPs by the SBP-ProG could rapidly be achieved without any chemical treatment. The immunosensor was fabricated through bonding of a partially gold-deposited cyclic olefin copolymer (COC) (top substrate) and gold patterned interdigitated array COC electrode (bottomsubstrate). To prevent electrode fouling, human immunoglobulin G (hIgG) was immobilized onto the ceiling inside the microchannel, instead of the bottom electrode. Alkaline phosphatase (AP)-labeled anti-hIgG was allowed to immunoreact with hIgG on the ceiling, followed by addition of an enzyme to generate an oxidative peak current. A three-fold increase in current was observed fromthe immunosensor without any electrode fouling compared with a control with the protein functionalized electrode. Also, the SiNPs facilely coated with AP-anti-hIgG via the SBP-ProG could increase the electrochemical signal up to 20% larger than that of the AP-anti-hIgG alone. Furthermore, this immunosensor was ultrasensitive with a detection limit of 0.68 pg/mL of a biomarker associated with prostate cancer. (C) 2017 Elsevier B. V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4439)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.