3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation
- Authors
- Kang, Dong-Ku; Gong, Xiuqing; Cho, Soongwon; Kim, Jin-young; Edel, Joshua B.; Chang, Soo-Ik; Choo, Jaebum; deMello, Andrew J.
- Issue Date
- 3-Nov-2015
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.87, no.21, pp 10770 - 10778
- Pages
- 9
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 87
- Number
- 21
- Start Page
- 10770
- End Page
- 10778
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45727
- DOI
- 10.1021/acs.analchem.5b02402
- ISSN
- 0003-2700
1520-6882
- Abstract
- Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating micro droplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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