Highly sensitive immunoassay of anti-cyclic citrullinated peptide marker using surface-enhanced Raman scattering detection
- Authors
- Chon, H.; Lee, S.; Wang, R.; Bang, S. -Y.; Lee, H. -S.; Bae, S. -C.; Hong, S. H.; Yoon, Y. H.; Lim, D.; Choo, Jaebum
- Issue Date
- 2015
- Publisher
- SPIE-INT SOC OPTICAL ENGINEERING
- Keywords
- Surface-enhanced Raman scattering; anti-cyclic citrullinated peptide antibody; rheumatoid arthritis; immunoassay; hollow gold nanospheres; magnetic bead; early diagnosis; blood sample
- Citation
- INTERNATIONAL CONFERENCE ON NANO-BIO SENSING, IMAGING, AND SPECTROSCOPY 2015, v.9523
- Journal Title
- INTERNATIONAL CONFERENCE ON NANO-BIO SENSING, IMAGING, AND SPECTROSCOPY 2015
- Volume
- 9523
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45770
- DOI
- 10.1117/12.2189760
- ISSN
- 0277-786X
1996-756X
- Abstract
- We report a highly sensitive anti-cyclic citrullinated peptide (anti-CCP) detection method for early diagnosis of rheumatoid arthritis (RA) using surface-enhanced Raman scattering (SERS)-based immunoassay. Herein, cyclic citrullinated peptide (CCP)-conjugated magnetic beads and anti-human IgG-conjugated hollow gold nanospheres (HGNs) were used as substrates and SERS nano-tags, respectively. First, its detection sensitivity was evaluated using anti-CCP standard solutions. Then quantitative anti-CCP levels, determined by the SERS-based assay, were compared with those obtained from three commercially available anti-CCP assay kits (Immunoscan CCPlus, ImmunnLisa(TM) CCP and BioPlex(TM) 2200) to assess its potential utility as a clinical tool. Finally, clinical samples from 20 RA patients were investigated using them. In the SERS-based assay, the anti-CCP level in human serum was successfully determined by monitoring the characteristic Raman peak intensity of SERS nano-tags. The diagnostic performance of our SERS-based immunoassay for clinical samples shows a good agreement with those measured by three commercial anti-CCP kits. In addition, our SERS-based assay results are more consistent in the low concentration range (0-25 U/mL) than those achieved by the commercial kits. Accordingly, it is estimated that the SERS-based assay is a potentially useful diagnostic tool for early diagnosis of RA.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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